Saccharomyces cerevisiae-Derived Mannan Does Not Alter Immune Responses to Aspergillus Allergens

Figure 6

Cytokine availability in the bronchoalveolar lavage (BAL) fluid of mice subjected to the fungal asthma model with and without MN treatment. All measured cytokines were increased in the BAL fluid after allergen exposure and challenge compared to baseline levels in the naïve controls (dashed lines). Interestingly, MN treatment alone resulted in the availability of IL-5, IL-1β, and TNFα in the airways (solid lines), while IL-4 was not affected by MN treatment. While MN treatment did not have an impact on cytokine production in allergic wild-type (WT) mice, transgenic (TG) mice had a further increase in IL-4 and IL-1β. Cytokine levels were higher in the MN-treated TGs than WT controls. Data are represented as the mean and standard deviation in -5 mice per group. Data in each group were analyzed by two-way ANOVA with Sidak’s multiple comparisons test. denotes , denotes , and denotes . Black bars denote WT mice while grey bars denote TG mice.