OCT4 Potentiates Radio-Resistance and Migration Activity of Rectal Cancer Cells by Improving Epithelial-Mesenchymal Transition in a ZEB1 Dependent Manner

Figure 1

OCT4 were positively associated with radio-resistance of human rectal cancer cells. (a) HT29 and SW480 cells were exposed to irradiation with indicated dose followed by another 24 hours incubation, and then cells were harvested and seeded 500 cells/well into six-well plate for 15-day incubation for clonogenic survival assay. Data are presented as mean ± SD, . versus control; versus control. (b) and (c) OCT4 protein expression and its variation during irradiation were detected by western blotting assay. Data are presented as mean ± SD, . versus control; versus control. (d) OCT4 mRNA expression and its variation during irradiation were detected by Real-Time PCR. Data are presented as mean ± SD, . versus control; versus control.

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