OCT4 Potentiates Radio-Resistance and Migration Activity of Rectal Cancer Cells by Improving Epithelial-Mesenchymal Transition in a ZEB1 Dependent Manner

Figure 5

ZEB1 was involved in OCT4 induced radio-resistance in human rectal cancer cells. (a) and (b) HT29 and SW480 cells were treated with indicated dose of radiation. ZEB1 protein expression and its variation during irradiation were detected by western blotting. (c) SW480 cells were transfected with OCT4 overexpression plasmid and the indicated proteins expression ((d) ZEB; (e) γ-HA2X) was determined in indicated times after 3Gy radiation treatment. (f) SW480 cells were transfected with OCT4 overexpression plasmid or the control for 24h. ZEB1 mRNA levels were detected by Real-Time PCR. Data are presented as mean ± SD, . versus control. (g) CHIP assay was performed using OCT4 antibody and corresponding IgG in SW480 cells after OCT4 overexpression plasmid transfection. Representative results from three independent experiments.

(a)

(b)

(c)

(d)

(e)

(f)

(g)