Purification and Characterization of Glutathione Binding Protein GsiB from Escherichia coli

Figure 4

Effects of GsiB deletion on cell growth and glutathione uptake. (a) GsiB and ggt gene deletion strains were constructed. The growth curves of mutant and wild type E. coli were recorded. pBAD24-gsiB was transformed into ΔGsiBΔggt to compensate for gene defection. (b) The effects of GsiB on glutathione import was determineted by recording glutathione concentration change in the medium, which was measured by Glutathione Assay Kit (Sigma). The glutathione uptake curves of mutant and wild type E. coli were analyzed.

(a)

(b)