Purification and Characterization of Glutathione Binding Protein GsiB from Escherichia coli

Table 1

Primers for gene expression, protein interaction and gene deletion.

Primer	Sequence 5‘ - 3’
GsiB-F	CATG CC ATGG CAAGAGCTGTACACCGTAG
GsiB-R	CCC AAGCTT ATTGCAAATCCGCGTCTTC
N-GsiA-F	CCATCTCGAG GCCACACAGTGATGAACTTGATG
N-GsiA-R	CGTCGGATCC TTATCTACGCATGAATGCGTATTCT
N-GsiB-F	CCATCTCGAG GGCAAGAGCTGTACACCGTAGTG
N-GsiB-R	CCATCTCGAG TTATTGCAAATCCGCGTCTTC
N-GsiC-F	CCATCTCGAG GCTTAATTACGTTATCAAACGCTTA
N-GsiC-R	CGTCGGATCC TTACTTGTACCTGATAGCCGGGTTA
C-GsiA-F	CATG CCATGG TGCCACACAGTGATGAACTTGATG
C-GsiA-R	CATGGACGTC CC TCTACGCATGAATGCGTATTCTG
C-GsiB-F	CATG CCATGG CAAGAGCTGTACACCGTAGTG
C-GsiB-R	CATGGACGTC CC TTGCAAATCCGCGTCTTCAAAG
C-GsiC-F	CATG CCATGG TGCTTAATTACGTTATCAAACGCT
C-GsiC-R	CATGGACGTC CC CTTGTACCTGATAGCCGGGTTA
C-GsiD-F	CATG CCATGG TG CGACTATTTAACTGGCGACG
C-GsiD-R	CATG CCATGG CC TCCTTTAATTTTCGGATCCAGC
GsiB-Del-F	GCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGC GTGTAGGCTGGAGCTGCTTC
GsiB-Del-R	AACAGCGTCGGAATCAACCCCAGTAAGCGTTTGATAACGTAATTAAGCATTCCACTCC CATATGAATATCCTCCTTAG
GGT-Del-F	CGATGATTAATTCAGAGTTATATACCAGGCTTAGCTGGGGTTGCCCCTTAATCTCTGGAG GTGTAGGCTGGAGCTGCTTC
GGT-Del-R	AGGCTACCTTCGGCTTGCCCTGACAAAATAGCCCTCTTCCCACGAAGAGGGCCGCTAACC CATATGAATATCCTCCTTAG
Del-F	GTGTAGGCTGGAGCTGCTTC
GsiB-Del-R	ACACCAGCACCGAGACGA
GGT-Del-R	GAACGGCAAAACCGCTGGA
GsiB-6His-R	CCC AAGCTT ACATCACCATCACCATCACTTGCAAATCCGCGT CTTCA