BioMed Research International / 2018 / Article / Tab 3

Research Article

Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHAC/PLGAs Scaffold on Osteogenesis

Table 3

Proliferative activity (OD value) and ALP activity (DEA enzyme activity, μmolmin−1g−1), relative expression of col1, Dlx-5, and osterix mRNA, relative expression of RUNX2, Dlx-5, osterix, and col1 protein of MC3T3-E1 cells in nHAC/PLGAs scaffold.

Time (days)Control groupVEGF groupBMP-2 groupBMP-2/VEGF group

Proliferative activity30.8507 ± 0.03171.3987 ± 0.1304##0.7385 ± 0.1160##1.1096 ± 0.0632

ALP activity74.2738 ± 0.84824.6884 ± 0.0979##29.8862 ± 1.4436##38.0940 ± 2.1503
144.3097 ± 0.88964.8018 ± 1.0833##47.5750 ± 3.0548##59.1847 ± 1.8423
214.0477 ± 1.17514.9057 ± 1.1357##54.8533 ± 2.9016##78.0085 ± 6.8198

Relative expression of col 1 mRNA71.0927 ± 0.07581.1907 ± 0.0939##1.3614 ± 0.2367##1.6776 ± 0.2768
141.1592 ± 0.10751.1095 ± 0.4211##1.5429 ± 0.1926##1.9635 ± 0.3183
210.9377 ± 0.07190.8569 ± 0.0447##2.5563 ± 0.3960##6.6649 ± 0.5074

Relative expression of col 1 protein70.0935 ± 0.02690.1288 ± 0.02010.1551 ± 0.02350.1908 ± 0.0840
140.0968 ± 0.00430.0963 ± 0.0277##0.3205 ± 0.0238##0.6859 ± 0.0372
210.1182 ± 0.02780.1310 ± 0.0254##0.4019 ± 0.0099##0.7932 ± 0.0635

Relative expression of RUNX2 protein70.0163 ± 0.00170.0258 ± 0.0082##0.0968 ± 0.00570.0762 ± 0.0158
140.0210 ± 0.00260.0294 ± 0.0073##0.1357 ± 0.02460.1401 ± 0.0260
210.0175 ± 0.00460.0292 ± 0.0064##0.1930 ± 0.08320.1500 ± 0.0386

Relative expression of Dlx-5 mRNA70.9742 ± 0.09211.0566 ± 0.0504##2.0851 ± 0.22932.1265 ± 0.4104
141.0388 ± 0.05150.9120 ± 0.3276##2.4911 ± 0.2984##3.6416 ± 0.7365
210.9527 ± 0.06440.8845 ± 0.0413##3.3096 ± 0.3557#4.5190 ± 1.3563

Relative expression of Dlx-5 protein70.0216 ± 0.01140.0348 ± 0.0012##0.1017 ± 0.02540.1161 ± 0.0230
140.0253 ± 0.00640.0218 ± 0.0036##0.0865 ± 0.0239#0.1506 ± 0.0289
210.0291 ± 0.00290.0447 ± 0.0109##0.2042 ± 0.0234##0.4216 ± 0.0362

Relative expression of osterix mRNA70.8809 ± 0.10610.7559 ± 0.0399#1.2075 ± 0.17321.3938 ± 0.4592
141.0333 ± 0.09240.8624 ± 0.2732##1.7024 ± 0.2401#2.1167 ± 0.4100
210.8365 ± 0.42780.6243 ± 0.0860##2.3860 ± 0.2616##6.9909 ± 1.6734

Relative expression of osterix protein70.0171 ± 0.00240.0153 ± 0.0045##0.0400 ± 0.0048#0.0814 ± 0.0182
140.0166 ± 0.00420.0209 ± 0.0028##0.0947 ± 0.0147##0.5140 ± 0.0287
210.0260 ± 0.00570.0243 ± 0.0036##0.2001 ± 0.0269##0.4832 ± 0.0376

Values are mean ± sd.P < 0.05 andP < 0.01 compared with control group. #P < 0.05 and ##P < 0.01 compared with BMP-2/VEGF group.

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