Enhancement of Immunoregulatory Function of Modified Bone Marrow Mesenchymal Stem Cells by Targeting SOCS1

Figure 1

Characteristics of rat BM-MSCs. (a) Morphology of MSCs at passages 1, 2, and 3. (b) Beta-galactosidase staining of MSCs at passage numbers 3 and 6. (c) Cell surface makers of MSCs, assessed by flow cytometry at passage 3. (d) Differentiated MSCs strained by Oil Red O (left) or Alizarin Red S (right). Adipocyte differentiation was induced and cells were strained by Oil Red O. Bone differentiation of MSCs was induced and cells were strained by Alizarin Red S (200x). (e) Coculture of BM-MSCs and spleen mononuclear cells (SMCs). The attached strip-like cells are BM-MSCs, while the floating cells are SMCs. Original magnifications ×200 for (a), (b), and (d).

(a)

(b)

(c)

(d)

(e)