Biochanin A Induces S Phase Arrest and Apoptosis in Lung Cancer Cells

Figure 5

Biochanin A induces apoptosis in A549 cells. Cells were incubated with Biochanin A (0, 50, 100, and 200μmol/L) for 48 h, followed by staining with annexin V/PI. The Q3 quadrant (annexin V−/PI−), Q4 quadrant (annexin V+/PI−), and Q2 quadrant (annexin V+/PI+) indicate the percentage of normal cells, early apoptosis, and late apoptosis, respectively. These results were from 1 representative experiment of 3 independent trials. Values represent the mean ± SD (n = 3). < 0.05; < 0.01; < 0.001.