Cell-Free Expression of Unnatural Amino Acid Incorporated Aquaporin SS9 with Improved Separation Performance in Biomimetic Membranes

Figure 2

Phylogenetic analysis of AQP SS9. The amino acid sequences of aquaporin homologs were aligned and the phylogenetic tree was constructed. TIP, a plant aquaporin (NCBI Accession Number P25818.1); MgaGlpF, Mycoplasma gallisepticum aquaglyceroporin (NCBI Accession Number ADC30962.1); MgeGlpF, Mycoplasma genitalium aquaglyceroporin (NCBI Accession Number AAC71249.1); BsGlpF, Bacillus subtilis aquaglyceroporin (NCBI Accession Number AOT51422.1); EcGlpF, E. coli aquaglyceroporin (NCBI Accession Number NP_418362.1); AQP1, an animal aquaporin (NCBI Accession Number P29972.3); 6083AqpZ, Synechocystis sp. PCC6803 aquaporin Z (NCBI Accession Number P73809.1); EcAqpZ, E. coli aquaporin Z (NCBI Accession Number NP_415396.1).