Cell-Free Expression of Unnatural Amino Acid Incorporated Aquaporin SS9 with Improved Separation Performance in Biomimetic Membranes

<div>Western-blot analysis of pPpa incorporated AQP SS9. Lane 1, pellet without pPpa; Lane 2, supernatant without pPpa; Lane 3, pellet with 2 μL pPpa; Lane 4, supernatant with 2 μL pPpa; Lane 5, pellet with 4 μL pPpa; Lane 6, supernatant with 4 μL pPpa; Lane 7, pellet with pPpa and pIVEX2.4c-MjpPpaRS; Lane 8, supernatant with pPpa and pIVEX2.4c-MjpPpaRS.</div>

BioMed Research International

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Figure 3

Figure 3: Cell-Free Expression of Unnatural Amino Acid Incorporated Aquaporin SS9 with Improved Separation Performance in Biomimetic Membranes 