Cell-Free Expression of Unnatural Amino Acid Incorporated Aquaporin SS9 with Improved Separation Performance in Biomimetic Membranes

Figure 5

Effect of fusion expression of P-AQP SS9 with different signal peptides. Lane 1, pIVEX2.4c-AQP SS9TAG; Lane 2, pIVEX2.4c-dsbAsp-AQP SS9TAG; Lane 3, pIVEX2.4c-malEsp-AQP SS9TAG; Lane 4, pIVEX2.4c-ompAsp-AQP SS9TAG; Lane 5, pIVEX2.4c-ompCsp-AQP SS9TAG; Lane 6, pIVEX2.4c-pelBsp-AQP SS9TAG; Lane 7, pIVEX2.4c-phoAsp-AQP SS9TAG; Lane 8, negative control without the supplementation of any AQP SS9 expression plasmid.