Cell-Free Expression of Unnatural Amino Acid Incorporated Aquaporin SS9 with Improved Separation Performance in Biomimetic Membranes

Box 1

Signal peptides used in this study to enhance the expression of AQP SS9 in cell-free system. The peptidase cleavage sites Ala-X-Ala were underlined.

dsbAsp (periplasmic protein disulphide isomerase I)

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser Ala Ser Ala

malEsp (maltose transporter subunit)

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr Thr Met Met Phe Ser Ala Ser Ala Leu Ala

ompAsp (outer membrane protein A)

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala Thr Val Ala Gln Ala

ompCsp (outer membrane protein C)

Met Lys Val Lys Val Leu Ser Leu Leu Val Pro Ala Leu Leu Val Ala Gly Ala Ala Asn Ala

pelBsp (pectate lyase B precursor)

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala Ala Gln Pro Ala Met Ala

phoAsp (alkaline phosphatase)

Met Lys Gln Ser Thr Ile Ala Leu Ala Leu Leu Pro Leu Leu Phe Thr Pro Val Thr Lys Ala