In Vitro Activity of β-Lactams in Combination with β-Lactamase Inhibitors against Mycobacterium tuberculosis Clinical Isolates

Table 2

Primers and conditions used for amplification and sequencing.

Primers	Sequence (5′-3′)	PCR conditions			Product length (bp)
		(s)	Annealing (°C, s)	(s)
BlaC-F	ATGCGCAACAGAGGATTCGGTC	30	61, 30	65	924
BlaC-R	CTATGCAAGCACACCGGCAACG
-F	ATGCGTCGAGTGGTTCGTTATC	30	58, 30	55	756
-R	CTAGCCGACCACCTCAATGG
-F	ATGCCAAAGGTGGGGATTGC	30	59, 30	90	1227
-R	TTACGCCTTGGCGTTACCGGC
DacB2-F	ACCAGCAACTGCTGGATTTC	30	60, 30	85	1196
DacB2-R	CGTTGATGACCAACGTCTTC
CrfA-F	ACCCGGCTCACAGAGAATCG	30	60, 30	40	457
CrfA-R	TATCACCGGTAGGCCATGC

Note: denaturation temperature was 94°C; elongation temperature was 72°C. All PCRs were performed for 31 cycles and each reaction was initialized by denaturation at 94°C for 10 min and terminated with a final elongation step at 72°C for 10 min.