The Potential Virulence Factors of Providencia stuartii: Motility, Adherence, and Invasion

Figure 5

Confocal microscopy of P. stuartii NK invasion into HeLa-M cells by confocal microscopy. Subconfluent HeLa-M monolayers were infected with P. stuartii NK at a ratio of 50 bacterial cells to 1 eukaryotic cell for 2 hours at 37°C. (a) Control (HeLa-M cells were not infected with bacteria); (b) Experiment (HeLa-M cells were infected with bacteria for 120 minutes). Actin cytoskeleton was stained with rhodamine-phalloidin (red); DNA of cell nuclei and bacteria were stained with DAPI (blue). Inset shows intracellular bacteria (white arrows).

(a)

(b)