Artificial Neural Network to Predict Varicocele Impact on Male Fertility through Testicular Endocannabinoid Gene Expression Profiles

Table 1

Primer sequences used for ECS gene expression profiling.

Target	Forward primer	Reverse primer	Efficiency
NAPE-PLD	5′-TGTCCCGGGTTCCAAAGAGGAGC-3′	5′-ACCATCAGCGTCGCGTGTCC-3′	1.94
FAAH	5′-ATGGAAGTCCTCCAAGAGC-3′	5′-TAGAGCTTTCAGGCATAGCG-3′	1.95
DAGLα	5′-ATTCTCTCCTTCCTCCTGC-3′	5′-ATTTGGGCTTGGTGCTTCG-3′	1.91
MAGL	5′-ATGTTGAAGAGGCTGGACATGC-3′	5′-ATGCAGATTCCGGATTGGC-3′	1.94
CB1	5′-TTCCACCGTAAAGACAGCCC-3′	5′-TCCACATCAGGCAAAAGGCC-3′	1.96
CB2	5′-TTGACCGATACCTATGTCTGTGC-3′	5′-TGCTTTCCAGAGGACATACCC-3′	1.92
TRPV1	5′-ATTGAACGGCGGAACATGACG-3′	5′-ATCTCTTCCAGCTTCAGCG-3′	1.97
GAPDH	5′-AGACAGCCGCATCTTCTTGT-3′	5′-CTTGCCGTGGGTAGAGTCAT-3′	1.99