Chronic Intake of Commercial Sweeteners Induces Changes in Feeding Behavior and Signaling Pathways Related to the Control of Appetite in BALB/c Mice

Figure 3

JAK2/STAT3, Akt, and SOCS expression in the brains of sweetener-supplemented male mice. Total brain protein extracts obtained at the end of the study were used to assess total and phosphorylated JAK2, STAT3, and Akt, as well as SOCS3, in male mice by western blot. β-actin was used as a loading control (a). male animals per group. One representative experiment of six is shown. (b) Densitometry analysis (average of six different experiments), expressed in mean of arbitrary units ± SEM. One-way analysis of variance was performed. compared to control group.