Papain Ameliorates the MPAs Formation-Mediated Activation of Monocytes by Inhibiting Cox-2 Expression via Regulating the MAPKs and PI3K/Akt Signal Pathway

Figure 4

Impact of TNF- on the inhibitory effects of papain on Cox-2 and MCP-1 expression of activated monocytes. (a) Transcriptional level of Cox-2 was measured by qRT-PCR detection in control, thrombin-induced, and papain, NS-398 as well as TNF-α-treated groups. (b) The levels of MCP-1 in the five groups were measured by ELISA kit. K-S test showed that data were in normal distribution in all groups (P>0.05). indicates thrombin-induced group versus control group. ^# indicates papain and thrombin-induced or NS-398-treated group versus only thrombin-induced group. indicates papain+TNF-α-treated group versus papain or NS-398-treated groups. p<0.01, ^##p<0.01, p<0.01.

(a)

(b)