Gossypol Promotes Bone Formation in Ovariectomy-Induced Osteoporosis through Regulating Cell Apoptosis

Figure 1

Gossypol promotes bone formation in ovariectomy-induced osteoporosis in vivo. (a) Mice underwent ovariectomy in the presence or absence of gossypol injection and the thighbone of each mouse was collected for H&E staining. Red arrow means trabeculae of epiphyseal and black arrow means trabeculae in metaphyseal regions. (b) Serum osteocalcin level of each mouse in control and gossypol injection groups was determined with Elisa methods. (c) Serum OPG levels were assessed with Elisa methods for the mice injected with solvent or gossypol. (d) Serum RANKL levels of mice from two groups were measured with Elisa methods. (e) Serum RANKL/OPG levels were assessed by the ratio of serum RANKL and serum OPG levels. Each experiment was repeated for three times. Data were presented as mean ±SEM. P<0.05 versus control.

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