Combination of Mitochondrial and Plasma Membrane Citrate Transporter Inhibitors Inhibits De Novo Lipogenesis Pathway and Triggers Apoptosis in Hepatocellular Carcinoma Cells

Figure 4

Effect of citrate transporter inhibitors on mitochondrial membrane potential (ΔΨm) in HepG2 cells. After 24 h, cells were incubated with CTPi, PMCTi, and the combination of 0.4 mM of each inhibitor with IC₅₀ concentrations. (a) ΔΨm in HepG2 cells was detected by flow cytometry and is shown in representative dot plot analysis. (b) Percentage of the loss of ΔΨm is shown in a bar graph. The control was defined as a vehicle for which cells were treated with a medium containing 0.2% DMSO alone. The CCCP was used as a positive control of the loss of ΔΨm. Data from at least three dependent experiments are expressed as mean ± SEM, .

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