Combination of Mitochondrial and Plasma Membrane Citrate Transporter Inhibitors Inhibits De Novo Lipogenesis Pathway and Triggers Apoptosis in Hepatocellular Carcinoma Cells

Figure 7

Effect of citrate transporter inhibitors on ΔΨm and activity of CPT-1 in HepG2 cells. (a) The loss of ΔΨm was determined by JC-1 staining and detected by flow cytometry. Cells were treated with 10 μg/ml of TOFA for 24 h, pretreated with 10 μg/ml of TOFA for 1 h followed by treatment with CTPi, PMCTi, and combined CTPi and PMCTi for 24 h. (b) Percentage of CPT-1 activity was determined in cells treated with each inhibitor and combined inhibitors and 0.1 mM of C75 for 24 h. The control was defined as a vehicle for which cells were treated with a medium containing 0.2% DMSO alone. Data are presented as mean ± SEM of three independent experiments, .

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