Twist Expression in Circulating Hepatocellular Carcinoma Cells Predicts Metastasis and Prognoses

Figure 2

CTCs detected in a blood sample from a HCC patient under the automated imaging fluorescent microscope. Note. (a) Representative images of CTCs stained for epithelial biomarker EpCAM and CK8/18/19 (red dots). (b) Representative images of CTCs stained for the mesenchymal biomarker Twist (green dots). (c) Representative images of CTCs stained for epithelial biomarker EpCAM, CK8/18/19 (red dots), and the mesenchymal biomarker Twist (green dots). (d) Representative images of negative control and leukocytes stained for CD45 expression (bright white fluorescence). The cells were analyzed using a 100x oil objective. CTCs: circulating tumor cells; HCC: hepatocellular carcinoma; EpCAM: epithelial cell adhesion molecule; CK: cytokeratin.

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