PPARα Regulates the Proliferation of Human Glioma Cells through miR-214 and E2F2

Figure 5

MiR-214 inhibits their tumorigenesis in nude mice via suppression of E2F2. (a) Bioinformatics prediction of miR-214 binding to the 3′-UTR of E2F2. (b) Luciferase reporter assay showed that miR-214 targets the 3′-UTR of E2F2 (versus control). (c) Western blot analysis of E2F2 protein levels. (d) CCK-8 assay of proliferation of U87/miR-214 cells after transfection with an E2F2 expression plasmid. (e) Tumorigenic capacity of stable cell lines in nude mice. (f) Statistical analysis of tumor volumes. (g) Western blot analysis of E2F2 protein expression in tumors excised from nude mice. Experiments were repeated in triplicate; ; .

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