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BioMed Research International
Volume 2018, Article ID 3849760, 9 pages
Research Article

Baicalein Accelerates Tendon-Bone Healing via Activation of Wnt/β-Catenin Signaling Pathway in Rats

1Department of Orthopedics, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510515, China
2Department of Spine Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China
3Department of Pain, Yue Bei People’s Hospital, Shaoguan, Guangdong 512000, China
4Department of Orthopedics, The First People’s Hospital of Guangyuan, Guangyuan, Sichuan 628017, China
5Department of Sports Medicine and Rehabilitation, Peking University Shenzhen Hospital, Shenzhen 518000, China

Correspondence should be addressed to Tao Li; moc.nuyila@oatilekug, Zhongmin Zhang; moc.nuyila@gnahznimgnohz, and Xintao Zhang; moc.anis@oatnixgnahz

Xinggui Tian, Huaji Jiang, and Yuhui Chen contributed equally to this work.

Received 11 March 2017; Accepted 26 July 2017; Published 6 March 2018

Academic Editor: Antoni Camins

Copyright © 2018 Xinggui Tian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Tendon-bone healing is a reconstructive procedure which requires a tendon graft healing to a bone tunnel or to the surface of bone after the junction injury between tendon, ligament, and bone. The surgical reattachment of tendon to bone often fails due to regeneration failure of the specialized tendon-bone junction. Materials and Methods. An extra-articular tendon-bone healing rat model was established to discuss the effect of the baicalein 10 mg/(kg·d) in accelerating tendon-bone healing progress. Also, tendon-derived stem cells (TDSCs) were treated with various concentrations of baicalein or dickkopf-1 (DKK-1) to stimulate differentiation for 14 days. Results. In vivo, tendon-bone healing strength of experiment group was obviously stronger than the control group in 3 weeks as well as in 6 weeks. And there were more mature fibroblasts, more Sharpey fibers, and larger new bone formation area treated intragastrically with baicalein compared with rats that were treated with vehicle for 3 weeks and 6 weeks. In vitro, after induction for 14 days, the expressions of osteoblast differentiation markers, that is, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osterix (OSX), and collagen I, were upregulated and Wnt/β-catenin signaling pathway was enhanced in TDSCs. The effect of DKK-1 significantly reduced the effect of baicalein on the osteogenic differentiation. Conclusion. These data suggest that baicalein may stimulate TDSCs osteogenic differentiation via activation of Wnt/β-catenin signaling pathway to accelerate tendon-bone healing.