Development of Two Analytical Methods Based on Reverse Phase Chromatographic and SDS-PAGE Gel for Assessment of Deglycosylation Yield in -Glycan Mapping

Figure 1

Method of quantification in level of deglycosylation with SDS-PAGE gel assay and GelAnalyzer software for a sample glycoprotein (fetuin). From left to right, numbers represent 1 (marker ladder), 2-b (glycosylated protein), 3-c (deglycosylated protein, 1 min), 4-d (deglycosylated, 3 min), 5-e (deglycosylated, 5 min), 6-f (deglycosylated, 8 min), and 7-g (deglycosylated, 10 min).