Development of Two Analytical Methods Based on Reverse Phase Chromatographic and SDS-PAGE Gel for Assessment of Deglycosylation Yield in -Glycan Mapping

Figure 5

Chromatogram of dextran ladder (a), N-glycan profile of NIST mAb prepared with GlycanAssure HyPerformance APTS kit and analyzed on Accucore 150 Amide HILIC LC Column and Vanquish Horizon UHPLC equipped with FLD zoomed out (b) and zoomed in views (c).

(a)

(b)

(c)