Research Article

Application of Autochthonous Lactobacillus Strains as Biopreservatives to Control Fungal Spoilage in Caciotta Cheese

Table 3

Evolution of pH and viable counts (log cfu/g) of moulds, lactococci, and lactobacilli in cheeses produced with (Lb and LBMix) and without (Reference) antifungal cultures, in trial T2 (cheese-surface inoculation of spores) during ripening at 8°C.

Cheese typeP. chrysogenum  ATCC 9179A. flavus ATCC 46283Control cheeses
Days of ripening
153015301530

MouldsReference8.80 ± 0.289.30 ± 0.428.60 ± 0.429.80 ± 0.283.54 ± 0.344.50 ± 0.28
Lb6.50 ± 0.718.30 ± 0.427.15 ± 0.118.74 ± 0.373.15 ± 0.114.23 ± 0.11
LbMix7.30 ± 0.428.10 ± 0.147.20 ± 0.577.41 ± 0.282.80 ± 0.283.80 ± 0.28

Presumptive lactococciReference7.40 ± 0.017.69 ± 0.017.46 ± 0.047.66 ± 0.027.13 ± 0.327.29 ± 0.40
Lb7.37 ± 0.017.60 ± 0.027.37 ± 0.047.61 ± 0.017.00 ± 0.567.30 ± 0.42
LbMix7.39 ± 0.017.61 ± 0.277.38 ± 0.017.60 ± 0.017.21 ± 0.297.30 ± 0.27

Presumptive lactobacilliReference<2<2<2<2<2<2
Lb7.88 ± 0.167.25 ± 0.077.44 ± 0.217.49 ± 0.157.65 ± 0.497.89 ± 0.58
LbMix8.04 ± 0.138.47 ± 0.018.32 ± 0.039.37 ± 0.048.13 ± 0.258.91 ± 0.13

pHReference5.68 ± 0.115.58 ± 0.115.53 ± 0.115.25 ± 0.075.91 ± 0.015.82 ± 0.03
Lb5.10 ± 0.145.05 ± 0.075.18 ± 0.044.85 ± 0.075.77 ± 0.035.68 ± 0.11
LbMix4.98 ± 0.024.78 ± 0.024.95 ± 0.074.74 ± 0.084.94 ± 0.034.90 ± 0.01

Values are the mean ± standard deviation of duplicate samples for each cheese type.
Different superscripts in the same column indicate significant differences (P < 0.05).
Uninoculated cheese samples: values are mean ± standard deviation of two aliquots of the same cheese sample.
Aflatoxin B1 producer.