FNDC4 Inhibits RANKL-Induced Osteoclast Formation by Suppressing NF-κB Activation and CXCL10 Expression

Figure 1

FNDC4 inhibited osteoclast formation in a dose-dependent manner. BMMs were cultured with different concentrations of FNDC4 (0, 100, 500, and 1000 ng/mL) in the presence of M-CSF (30 ng/mL) and RANKL (100 ng/mL) for 5 days. (a) Representative images of TRAP-stained cells treated with different concentrations of FNDC4 and TRAP-positive multinucleated cells with more than three nuclei were counted as osteoclasts. (b) Quantification of TRAP-positive osteoclasts showed that FNDC4 at 500 ng/mL and 1000 ng/mL could significantly reduce the number of TRAP-stained osteoclasts. (c) CCK-8 analysis was conducted to assess the effect of FNDC4 on the proliferation and viability of BMMs. All experiments were performed in triplicate and results were presented as ;   and relative to the RANKL group.

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