FNDC4 Inhibits RANKL-Induced Osteoclast Formation by Suppressing NF-κB Activation and CXCL10 Expression

Figure 6

FNDC4 inhibited the osteoclast formation via downregulation of CXCL10. (a) FNDC4 could significantly suppress the expression of CXCL10 in BMMs. BMMs were treated with M-CSF (30 ng/mL) and RANKL (100 ng/mL) in the presence of absence of 1000 ng/mL FNDC4 for 2 days; then total RNA was collected for qPCR analysis. (b) BMMs were treated with M-CSF (30 ng/mL) and RANKL (100 ng/mL) in the presence of absence of 1000 ng/mL FNDC4 for 2 days; then the medium was collected to detect the concentration of CXCL10 using ELISA. (c) The selective silencing of CXCR3 was confirmed by western blotting; si#3 was selected for later experiments. (d, e) The addition of CXCL10 could attenuate the FNDC4-induced inhibition of osteoclast formation. BMMs were incubated with RANKL (100 ng/mL), M-CSF (30 ng/mL), and FNDC4 (1000 ng/mL) with or without the supplementation of CXCL10 (10 ng/mL) and siRNA against CXCR3 for 5 days. Representative images of TRAP-stained cells and quantitative analysis of TRAP-positive cells were presented for three independent experiments. Data were presented as . and .

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