Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness

Figure 1

Substrates with varying stiffness were fabricated by tuning the ratios of Sylgard 184 mixed with Sylgard 527 (from 1:0, 5:1, and 1:1 to 1:3). (a) Representative stress–strain curves for four different PDMS formulations measured by biomechanical testing. (b) Elastic modulus of the four different substrates obtained from the load–distance curve.

(a)

(b)