Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness

Figure 3

Focal adhesion was regulated by substrate stiffness. (a) Immunofluorescent images of vinculin in MC3T3-E1 plated on different substrates (ranging from 1:0 to 1:3, elastic modulus ranging from 2.7 MPa to 0.6 MPa) (scale bar = 50 μm). (b) The protein expression level of vinculin detected by western blotting and quantitative analysis. Vinculin results were normalized to ACTB. p < 0.05 vs. 1:0, #p < 0.05 vs. 5:1, p< 0.05 vs. 1:1.

(a)

(b)