Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness

Figure 4

Osteogenesis capacity of MC3T3-E1 was altered by substrate stiffness. (a) The expression of osteoblast markers OPN, OCN, and Col I measured by western blot analysis after 3 and 4 weeks of culture on different substrates. (b) Alizarin red staining of mineral nodule formation on the substrates of cells cultured for 4 weeks and quantitative analysis of dissolved color by reading the absorbance at 562 nm. p < 0.05 vs. 1:0, #p < 0.05 vs. 5:1, p< 0.05 vs. 1:1.

(a)

(b)