Research Article
Distinct Epitopes on CD13 Mediate Opposite Consequences for Cell Adhesion
Figure 9
Monoclonal anti-CD13 antibodies distinctively inhibit binding of HEK-ANPEP cells to fibronectin. HEK-ANPEP-GFP cells (HEK cells expressing a recombinant CD13 fused to GFP) were incubated with the indicated antibodies (or a control IgG) for 30 minutes at 37°C and transferred to fibronectin-sensitized plates to allow adherence for 15 min at 37°C. Cells were washed very gently and cells attached to the plate were quantitated by measuring the fluorescence in each well with the Cytation 3 plate reader. Data is shown as percentages compared to the number of control cells (without antibody added) attached to similar wells. Average ± SD of three experiments with each condition assayed in triplicate. .
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