Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages

Figure 3

B. cenocepacia entry into macrophages requires macropinocytosis induction and contribution of the T3SS. Macrophages were seeded onto 24-well culture plates and infected with a MOI of 100. (a) Bacterial adherence to macrophages at 4°C during 1 h in the presence of the vehicle (mock), wortmannin (Wo), or cytochalasin B (CB). (b) Percentage of the bacterial entry in mock cells or in presence of Wo or CB at 37°C during 1 h. (c) Invasion index of B. cenocepacia and the mutant in the ΔT3SS to macrophages at 30 min after infection. NS, nonsignificant; .

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