Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages

Figure 4

B. cenocepacia entry induces macropinosome formation. Macrophages were infected with B. cenocepacia-pDSRedT3 at a MOI of 100 in presence of the phase-fluid Dextran-FITC 10 kDa. (a) Representative image of macrophages infected with B. cenocepacia-pDSRedT3 in presence of the phase-fluid and analyzed by immunofluorescence. (b) Quantification of macropinosomes containing the phase-fluid marker and B. cenocepacia-RFP or B. cenocepacia-ΔT3SS-RFP. (c) Quantification by flow cytometry of the phase-fluid uptake of uninfected cells (mock) or infected cells with B. cenocepacia-pSDRedT3. The arrow in the inset (a) indicates the bacterial localization into a macropinosome. Values represent the mean of SEM from three independent experiments. NS, nonsignificant; ; . The scale bar represents 10 μm.

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