Burkholderia cenocepacia Induces Macropinocytosis to Enter Macrophages

Figure 5

B. cenocepacia moves freely into a macropinosome. Macrophages were placed in a 35-mm Petri dish coupled to a 14-mm microwell and observed under a 60x lens with phase-contrast optics; an image was taken every 30 s during 25 min. Cells were infected for 15 min; then, we documented the B. cenocepacia interaction with macrophages for an additional 20 min (900 to 2000 s). The white arrow indicates the B. cenocepacia localization into a macropinosome. The bar indicates 10 m.