Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2018 (2018), Article ID 4315861, 9 pages
https://doi.org/10.1155/2018/4315861
Research Article

Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line

1Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China
2Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, Jinan 250100, China
3Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802, USA
4Shandong Provincial Center for Animal Disease Control and Prevention, Jinan 250022, China
5Department of Biology and Microbiology and Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA

Correspondence should be addressed to Jinbao Wang; nc.ca.saas@bjgnaw, Xiaomin Zhao; moc.361@66oahzmx, and Yijun Du; moc.361@6190nujiyud

Received 14 October 2017; Revised 18 December 2017; Accepted 1 January 2018; Published 21 February 2018

Academic Editor: Yanjin Zhang

Copyright © 2018 Xiangju Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Isolation and identification of diverse porcine reproductive and respiratory syndrome viruses (PRRSVs) play a fundamental role in PRRSV research and disease management. However, PRRSV has a restricted cell tropism for infection. MARC-145 cells are routinely used for North American genotype PRRSV isolation and vaccine production. But MARC-145 cells have some limitations such as low virus yield. CD163 is a cellular receptor that mediates productive infection of PRRSV in various nonpermissive cell lines. In this study, we established a high and stable porcine CD163- (pCD163-) expressing MARC-145 cell line toward increasing its susceptibility to PRRSV infection. Indirect immunofluorescence assay (IFA) and Western blotting assays showed that pCD163 was expressed higher in pCD163-MARC cell line than MARC-145 cells. Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. Finally, we found that pCD163-MARC cell line had a higher isolation rate of clinical PRRSV samples and propagated live attenuated PRRS vaccine strains more efficiently than MARC-145 cells. This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV.