Hsp90 Inhibition Reduces TLR5 Surface Expression and NF-κB Activation in Human Myeloid Leukemia THP-1 Cells

Figure 5

Inhibition of Hsp90 reduces total protein levels of TLR5. THP-1 cells were treated with or without the Hsp90 inhibitor GA (20, 50, 100, 200, and 500 nM) for 24 hr. The levels of TLR5 protein expression were measured by intracellular flow cytometry (a) or Western blot (b). Shown are representative blots and densitometric ratios of proteins normalized to β-actin. Data are expressed as means of relative expression ratio ± SD ( or 4). versus DMSO-treated group.

(a)

(b)