Hsp90 Inhibition Reduces TLR5 Surface Expression and NF-κB Activation in Human Myeloid Leukemia THP-1 Cells
Inhibition of Hsp70 enhances TLR5 mRNA expression, while reducing TLR5 surface expression. (a) The Hsp70 inhibitor VER155008 enhances TLR5 mRNA expression in THP-1 cells. Gene expression of TLR5 was determined by qRT-PCR and was normalized to that of β-actin. THP-1 cells were treated with or without 20 μM VER155008 for 24 h. Data are expressed as means of relative expression ratio ± SD (). versus DMSO-treated group. (b) Cell surface expression of TLR5 in THP-1 cells was examined by flow cytometry analysis. THP-1 cells were treated with or without VER155008, at various concentrations, for 24 h. Relative fluorescence intensity was measured, and data are expressed as relative TLR5 expression ± SD (). versus DMSO-treated group.
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