Insight into the Hydrolytic Selectivity of β-Glucosidase to Enhance the Contents of Desired Active Phytochemicals in Medicinal Plants

Figure 2

Enzymatic conversion of the glycosides liquiritin, geniposide, baicalin, glycyrrhizin, hesperidin, neohesperidin, and naringin using recombinant β-glucosidase from Lactobacillus anti (rBGLa). Each glycoside (1 mM) (pH 6.0) was incubated with 100 μg/mL rBGLa at 45°C for 17 h, and the concentration of samples withdrawn at 0, 1, 2, 4, 8, and 17 h after the start of the reaction was quantified by HPLC analysis in triplicate.