Insight into the Hydrolytic Selectivity of β-Glucosidase to Enhance the Contents of Desired Active Phytochemicals in Medicinal Plants

Figure 3

Sequence alignments of recombinant β-glucosidase from Lactobacillus anti (rBGLa) with glycoside hydrolase family 3 (GH3) β-glucosidase from Bifidobacterium adolescentis (BaBgl3, PDB code 5WAB), Kluyveromyces marxianus (KmBglI, PDB code 3ABZ), and Thermotoga neapolitana (TnBgl3B, PDB code 2X40). BaBgl3, KmBglI, and TnBgl3B were selected by global model quality estimation scores derived from modeling of rBGLa through the Swiss-Model. The functional residues predicted are indicated in red (catalytic reaction) and green (substrate specificity) boxes.