BioMed Research International

Research Article

Insight into the Hydrolytic Selectivity of β-Glucosidase to Enhance the Contents of Desired Active Phytochemicals in Medicinal Plants

Table 2

Relative specific activities and enzyme kinetics of β-glucosidase from Lactobaillus antri (rBGLa).


Chemicals	Relative activity ()	Kinetic parameter
Chemicals	Relative activity ()	kcat (s^-1)	K_m (mM)	kcat/K_m (mM^-1 s^-1)

pNPG	100 ± 1	407 ± 7	1.84 ± 0.07	221 ± 9

pNPG + 0.1mM cellobiose	57.3 ± 1.5	432 ± 32	4.40 ± 0.57	98.1 ± 14.7

pNPGr	0.00123 ± 0.00006	0.213 ± 0.005	102 ± 6	0.00209 ± 0.00012

Liquiritin	37.1 ± 0.3	-	-	-

Geniposide	2.20 ± 0.04	-	-	-

Baicalin	0.0261 ± 0.0002	-	-	-

Glycyrrhizin	0.000190 ± 0.000103	-	-	-

Hesperidin	0.0199 ± 0.0005	-	-	-

Neohesperidin	0.00120 ± 0.00004	-	-	-

Naringin	0.000866 ± 0.000090	-	-	-

pNPG, p-nitrophenyl β--glucopyranoside; pNPGr, p-nitrophenyl β--glucuronide.
For the measurement of relative activities, 1 mM substrates were reacted with 100 μg/mL rBGLa (pNPG, pNPGr, baicalin, glycyrrhizin, hesperidin, and naringin) and 0.1 μg/mL rBGLa (liquiritin and geniposide).