Candidate Genes as Biomarkers in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome Based on mRNA Expression Profile by Next-Generation RNA-Seq Analysis

Figure 1

Differential expression of genes in lung tissues (fold change > 1.5, and value < 0.05). (a) Hierarchical clustering analysis of genes that were differentially expressed in lung tissue samples between LPS-induced ARDS (group-L) and normal rat (group-C); each group contains 3 individuals. Green-black indicates lower expression, and red indicates high expression. (b) The scatter plot is used for evaluating the changing expression profiles of genes between group-L and group-C tissue samples. The values corresponding to the - and -axes in the scatter plot are the normalized signal values of the samples ( scaled). The diagonal dotted lines represent fold changes. The genes above the top green line and below the bottom green line represent the differential expression genes. (c) The differentially expressed genes (shown in red font) with statistical significance from lung tissues between group-C and group-L screened using a volcano plot. The vertical line represents a boundary of the differential and nondifferential expression genes, and the horizontal line corresponds to a value equal to 0.05.

(a)

(b)

(c)