Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human Osteoblasts In Vitro Treated with Bisphosphonates

Figure 4

Cumulative bone morphogenetic protein-2 (BMP-2, pg) release after 3 days (a) and 21 days (b) of culture. (a) The cells were cultured in complete medium (OGM) or Concentrated Growth Factor (CGF), which represents the controls, and in presence of resveratrol 10 μM (R10), alendronate 5 μM (AL5), zoledronate 5 μM (ZOL5), alendronate 5 μM and resveratrol 10 μM (AL5 + R10), and zoledronate 5 μM and resveratrol 10 μM (ZOL5 + R10). The values are reported as total amount of BMP-2 released ± SE, < 0,05 and in particular < 0,05 versus OGM; < 0,05 versus CGF; ^▴ < 0,05 versus OGM + R10; < 0,05 versus CGF + R10. (b) The cells were cultured in mineralization medium (OMM) also supplemented with Concentrated Growth Factor (OMM + CGF), which represents the controls, and in presence of resveratrol 10 μM (R10), alendronate 5 μM (AL5), zoledronate 5 μM (ZOL5), alendronate 5 μM and resveratrol 10 μM (AL5 + R10), and zoledronate 5 μM and resveratrol 10 μM (ZOL5 + R10). The values are reported as total amount of BMP-2 released ± SE, < 0,05 and in particular < 0,05 versus OMM; < 0,05 versus OMM + CGF; ^▴ < 0,05 versus OMM + R10; < 0,05 versus OMM + CGF + R10.

(a)

(b)