Beneficial Effects of Concentrated Growth Factors and Resveratrol on Human Osteoblasts In Vitro Treated with Bisphosphonates

Figure 5

Quantitative evaluation of sirtuin 1 (SIRT-1) immunopositivity in osteoblasts (HOB) after 3 days of culture as IOD (Integrated Optical Density). A micrograph as an example has been reported. The cells were cultured in complete medium (OGM) or Concentrated Growth Factor (CGF), which represents the controls, and in presence of resveratrol 10 μM (R10), alendronate 5 μM (AL5), zoledronate 5 μM (ZOL5), alendronate 5 μM and resveratrol 10 μM (AL5 + R10), and zoledronate 5 μM and resveratrol 10 μM (ZOL5 + R10). Data represent means ± SE, < 0,05 and in particular < 0,05 versus OGM; < 0,05 versus CGF; ^▴ < 0,05 versus OGM + R10; < 0,05 versus CGF + R10.