HSP-70-Mediated Hyperbaric Oxygen Reduces Brain and Pulmonary Edema and Cognitive Deficits in Rats in a Simulated High-Altitude Exposure

Figure 4

(a) Comparison of latency to entering the dark chamber before receiving the foot shock (preshock; acquisition time) and 24 h after receiving the foot shock (after 24 h; retention time). Each column and bar represents the mean±SD of 10 rats per group. The acquisition time did not significantly differ among the four groups. However, the retention time was significantly lower in the HAE+non-HBO₂T group compared with the non-HAE+non-HBO₂T controls at 5 days after injury (P<0.05). The HAE+HBO₂T group rats showed a significant increase of retention time in passive avoidance test (⁺P<0.05). However, the HAE+HSP-70 Ab+HBO₂T group rats showed a significant decrease of retention time in passive avoidance compared with the HAE+HBO₂T group (^#P<0.05). The Y-maze test evaluates learning and spatial memory function in rats 5 days after HAE. Data of both time of reaction (b) and error number (c) are presented as means±SD of n=10 per group. The Y-maze test results showed that the HAE animals had a deficit in spatial memory (time of reaction prolonged and error number increased) compared with the non-HAE+non-HBO₂T controls at 5 days after injury (P<0.05). The HAE+HBO₂T group rats showed a significant decrease of time of reaction and error number in Y-maze test with the HAE+non-HBO₂T group (⁺P<0.05). However, the HAE+HSP-70 Ab+HBO₂T group rats showed a significant increase of time of reaction and error number in Y-maze test compared with the HAE+HBO₂T group (^#P<0.05)

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