The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy

Figure 2

EAEG treatment did not induce apoptotic cell death in HeLa cells. (a) DNA fragmentation analysis. Lines a and e, controls treated for 24 and 48 h, respectively; lines b and f, 45 μg/ml EAEG treated for 24 and 48 h, respectively; lines c and g, 60 μg/ml EAEG treated for 24 and 48 h, respectively; lines d and h, 75 μg/ml EAEG treated for 24 and 48 h, respectively; line i, DNA marker. ((b) and (c)) The expression of Bid, Bax, Bcl-2, and caspase-3 in HeLa cells treated with different concentrations of EAEG (0, 45, 60, and 75 μg/ml) for 24 h (b) or 48 h (c). β-Actin served as the loading control. The relative protein/β-actin ratios were calculated using ImageJ. ((d) and (e)) Cell viability of HeLa cells treated with/without Z-VAD-FMK (50 μM) and different concentrations of EAEG for 24 h (d) or 48 h (e). The data were expressed as mean ± SEM (). EAEG: ethyl acetate extract of Gynura formosana Kitam. leaves.

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