The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy

Figure 3

EAEG induced autophagy in HeLa cells. (a) Analysis of GFP-LC3 puncta formation in GFP-LC3/HeLa cells under fluorescence microscopy. Cells were treated for 24 h with DMEM only (blank), EAEG solvent (DMSO) as control, different concentrations (45, 60, and 75 μg/ml) of EAEG, or the positive control trehalose (Tre) (scale bar = 20 μm.). (b) The expression analysis of the autophagy-related proteins p62 and LC3 by Western blot. HeLa cells were treated for 24 h with different concentrations (0, 45, 60, and 75 μg/ml) of EAEG. (c) The relative protein levels of p62 and LC3-II in HeLa cells treated with 60 μg/ml EAEG with or without CQ (50 mM) for 24 h. GAPDH served as the loading control. The protein relative ratio was calculated using ImageJ. The data were expressed as mean ± SEM (). and with respect to the control group. with respect to EAEG treatment group. EAEG: ethyl acetate extract of Gynura formosana Kitam. leaves.

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