The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy

Figure 4

The conversation of LC3-I to LC3-II. Protein level of p62 was measured by Western blot. (a) HeLa cells were treated with EAEG at a concentration of 0, 40, 50, 60, 70, or 80 μg/ml for 24 h. (b) HeLa cells were treated with 60 μg/ml EAEG for 0, 3, 6, 9, 18, or 24 h. GAPDH served as the loading control. The relative protein ratio was calculated using ImageJ. The data were expressed as mean ± SEM (). , , and with respect to the control group. EAEG: ethyl acetate extract of Gynura formosana Kitam. leaves.

(a)

(b)