MicroRNA-219-5p Promotes Tumor Growth and Metastasis of Hepatocellular Carcinoma by Regulating Cadherin 1

Figure 4

CDH1 is identified as a downstream target of miR-219-5p. (a) Sequences of CDH1 3′UTR and miR-219-5p according to the prediction of microRNA.org. Wild-type and mutated-type binding sequences of CDH1 3′UTR are shown. (b) Relative luciferase activity in HEK293T cells transfected with reporter vector containing wild-type or mutated-type binding sequence along with miR-219-5p mimic or negative control. CDH1 protein (c) and mRNA levels (d) in HCC cells treated with miR-219-5p mimic or antagomir. (e) Linear regression analysis between miR-219-5p and CDH1 in HCCs. (f) Working model for the role of miR-219-5p in regulation of CDH1 in HCC. Data are shown as mean ± SD. , .

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