A Novel Thermostable GH3 β-Glucosidase from Talaromyce leycettanus with Broad Substrate Specificity and Significant Soybean Isoflavone Glycosides-Hydrolyzing Capability

Table 1

Effects of metal ions and chemical reagents (5 mM) on the activity of purified recombinant Bgl3B.

Chemical	Relative activity (%)	Chemical	Relative activity (%)
Control	100.0 ± 1.5	Zn^2+	94.2 ± 1.5
K^+	100.1 ± 0.6	Mn^2+	89.3 ± 1.9
Mg^2+	98.3 ± 0.9	Cu^2+	83.1 ± 0.3
Pb^2+	98.1 ± 0.4	Fe^3+	5.2 ± 0.3
Ni^2+	97.8 ± 0.0	Ag^+	ND
Na^+	97.3 ± 0.3	β-Mercaptoethanol	95.6 ± 0.1
Ca^2+	96.8 ± 1.1	EDTA	89.8 ± 1.1
Cr^3+	96.2 ± 0.6	SDS	83.9 ± 0.3
Co^2+	94.9 ± 0.2

The data are shown as the mean ± SD (n = 3); ND, not detected.